Physical behavior of streptolysin S

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Stabilization of Streptolysin S by Potassium Ions

Potassium ions, and to a less extent, ammonium, magnesium, and barium ions, protect streptolysin S against thermal inactivation.

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Reactivation of streptolysin S by oligonucleotide.

Oligonucleotide-streptolysin S complex inactivated by alkali treatment remains nonhemolytic, even after acidification and mixing with intact carrier oligonucleotide rich in guanyl residue. Upon dehydration, however, the inactive streptolysin S-oligonucleotide mixture turned to be hemolytic, and this reactivation of the hemolysin was promoted by treatment with guanidine hydrochloride. After alka...

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The Purification and Properties of Streptolysin S

Considerable purification of streptolysin S has been achieved by fractionation of crude bacterial filtrates with methanol, under controlled conditions of pH, ionic strength, and temperature. The final material (P III) consisted of two electrophoretic components. The purified material was employed for kinetic studies, the results of which were confirmed with hemolysin isolated electrophoreticall...

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Streptolysin S activation by lipoteichoic acid.

Lipoteichoic acid from streptococci and Staphylococcus aureus both activated membrane-bound precursor streptolysin S and induced the formation of extracellular streptolysin S. Lipoteichoic acid could replace other substances, such as yeast ribonucleic acid core, that act as carriers for hemolysin but which are not components of the streptococcus or the host. Lipoteichoic acid may play a role as...

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Effects of polynucleotides on production and activity of streptolysin S.

Activity of various natural and synthetic polynucleotides as a carrier for streptolysin S was tested in a resting cell system. As the carrier, intact molecules of MS2 RNA, E coli tRNA or rat liver RNA were almost inactive, whereas RNase I core of these RNAs, especially the core fractions eluted from DEAE-cellulose column at higher NaCl concentrations, effectively induced production of the extra...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1967

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.93.6.2024-2025.1967